Kannagi M, Sugamura K, Kinoshita K, Uchino H, Hinuma Y
J Immunol. 1984 Aug;133(2):1037-41.
Cytotoxic T cells (Tc) derived from one patient with adult T cell leukemia/lymphoma (ATL) killed fresh autologous lymphoma cells in vitro. The Tc were induced from peripheral blood leukocytes (PBL) of this patient during remission by multiple in vitro stimulations with an autologous ATLV-bearing cell line (ILT) that was previously established by cloning of PBL in the presence of interleukin 2 (IL 2). PBL from eight other ATL patients were stimulated in the same manner, and responder cells from a patient in remission also showed cytotoxicity specific for ATL virus (ATLV)-bearing cells. Fresh lymphoma cells were obtained in relapse and were used as target cells for the autologous Tc induced. They became susceptible to the Tc within 4 hr of in vitro incubation, and their susceptibility increased with incubation time for at least 12 hr. ATLV antigens on the cell surface of these lymphoma cells, however, were not detected by radioimmunoassay during these incubation periods, but were detectable after 16 hr of incubation. In addition, cytotoxicity against lymphoma cells was completely inhibited by autologous ILT cells used as "cold" target competitor cells. These findings indicate that the target antigen of the Tc was expressed on both autologous ILT cells and lymphoma cells, and it may be different from ATLV antigens detected by serologic methods. In addition, the data suggested allogeneic restriction of the Tc in that the preferentially killed allogeneic ATLV-bearing cells share several HLA antigens.
从一名成人T细胞白血病/淋巴瘤（ATL）患者体内分离出的细胞毒性T细胞（Tc）在体外可杀死新鲜的自体淋巴瘤细胞。这些Tc细胞是在患者缓解期，通过用先前在白细胞介素2（IL-2）存在下克隆外周血白细胞（PBL）建立的自体携带ATLV的细胞系（ILT）进行多次体外刺激，从该患者的外周血白细胞中诱导产生的。另外8名ATL患者的PBL也以同样的方式进行刺激，一名缓解期患者的反应细胞也表现出对携带ATL病毒（ATLV）细胞的细胞毒性。复发时获取新鲜的淋巴瘤细胞，并将其用作诱导产生的自体Tc细胞的靶细胞。在体外培养4小时内，它们对Tc细胞变得敏感，并且其敏感性在至少12小时的培养时间内随时间增加。然而，在这些培养期间，通过放射免疫测定未检测到这些淋巴瘤细胞表面的ATLV抗原，但在培养16小时后可检测到。此外，用作“冷”靶竞争细胞的自体ILT细胞可完全抑制对淋巴瘤细胞的细胞毒性。这些发现表明，Tc细胞的靶抗原在自体ILT细胞和淋巴瘤细胞上均有表达，并且它可能与血清学方法检测到的ATLV抗原不同。此外，数据表明Tc细胞存在同种异体限制，因为优先杀死的同种异体携带ATLV的细胞共享几种HLA抗原。
