Stockinger H, Majdic O, Liszka K, Aberer W, Bettelheim P, Lutz D, Knapp W
J Natl Cancer Inst. 1984 Jul;73(1):7-11.
The 3-fucosyl-N-acetyllactosamine structure, a sugar sequence contained in the human milk oligosaccharide lacto-N-fucopentaose III, is recognized by most of the granulocyte-specific monoclonal antibodies (MoAb) reported in the literature, including the six MoAb from our laboratory. Blast cells from patients with acute myeloblastic leukemia (AML) displayed a heterogeneous reaction pattern when they were exposed to MoAb against this moiety, and the proportion of reactive cells in individual cell samples was highly variable. The intensity of the reaction was strongly enhanced by neuraminidase treatment of AML blasts, and reactive structures were exposed on previously negative AML blast cells. Surprisingly, this granulocyte-associated antigen was exposed by desialylation not only on malignant myeloid precursor cells but also on common acute lymphoblastic leukemia cells. No such effect was seen when normal peripheral blood lymphocytes, lymphocytes from patients with chronic lymphatic leukemia, or blast cells from patients with B-cell acute lymphoblastic leukemia, acute erythroid leukemia, and acute megakaryoblastic leukemia were treated with neuraminidase.
3-岩藻糖基-N-乙酰乳糖胺结构是母乳中低聚糖乳糖-N-岩藻五糖III所含的一种糖序列，文献报道的大多数粒细胞特异性单克隆抗体（MoAb）均可识别该结构，包括我们实验室的六种MoAb。急性髓细胞白血病（AML）患者的原始细胞在接触针对该部分的MoAb时呈现出异质性反应模式，且单个细胞样本中反应性细胞的比例差异很大。AML原始细胞经神经氨酸酶处理后，反应强度显著增强，且反应性结构在先前阴性的AML原始细胞上暴露出来。令人惊讶的是，这种与粒细胞相关的抗原不仅在恶性髓系前体细胞上通过去唾液酸化暴露出来，在常见的急性淋巴细胞白血病细胞上也有暴露。当正常外周血淋巴细胞、慢性淋巴细胞白血病患者的淋巴细胞或B细胞急性淋巴细胞白血病、急性红白血病和急性巨核细胞白血病患者的原始细胞用神经氨酸酶处理时，未观察到这种效应。
