Enea V, Ellis J, Zavala F, Arnot D E, Asavanich A, Masuda A, Quakyi I, Nussenzweig R S
Science. 1984 Aug 10;225(4662):628-30. doi: 10.1126/science.6204384.
A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS beta-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.
通过用针对环子孢子（CS）蛋白的单克隆抗体筛选大肠杆菌互补DNA文库，已分离出编码人类疟原虫恶性疟原虫CS蛋白的互补DNA克隆。互补DNA插入片段的DNA序列编码一个四氨基酸序列：脯氨酸-天冬酰胺-丙氨酸-天冬酰胺，串联重复23次。CSβ-内酰胺酶融合蛋白能特异性结合针对CS蛋白的单克隆抗体，并抑制这些抗体与天然恶性疟原虫CS蛋白的结合。这些发现为开发针对恶性疟原虫疟疾的疫苗提供了基础。
