Martinov S, Popov G
Vet Med Nauki. 1984;21(4):15-25.
Described is a modified method for the production of etherial-acetone antigens used in the complement-fixation test (CFT). It is characterized by a two-fold etherial extraction and an electron-microscopic control over the strains and the chlamydial suspensions to be used as initial material. The antigens obtained by the modified method are said to have high titers, stability, and high specific activity, are long-lasting, and do not produce anticomplement reactions. A new method has been worked out for the production of concentrated and purified chlamydial antigens for CFT, employing column chromatography on Sepharose 2B or 4B. The antigens obtained are highly active and specific. Results have shown that the use of the highly purified chlamydial antigens for CFT is very promising.
描述了一种用于制备补体结合试验（CFT）中使用的乙醚丙酮抗原的改良方法。其特征在于进行两次乙醚提取，并对用作起始材料的菌株和衣原体悬液进行电子显微镜检查。据说通过改良方法获得的抗原具有高滴度、稳定性、高比活性，持久且不产生抗补体反应。已经开发出一种新方法，使用琼脂糖2B或4B柱色谱法生产用于CFT的浓缩和纯化的衣原体抗原。获得的抗原具有高活性和特异性。结果表明，将高度纯化的衣原体抗原用于CFT非常有前景。
