French B T, Hanausek-Walaszek M, Walaszek Z, Schumm D E, Webb T E
Cancer Lett. 1984 May;23(1):45-52. doi: 10.1016/0304-3835(84)90060-0.
Treatment of rats with the hepatocarcinogen 3'-methyl-4-dimethylamino-azobenzene (3'-MeDAB) causes the appearance in the liver cytosol of a 60 kilodalton oncofetal protein. The appearance of this factor occurs within 40 h of treatment and coincides with the increase in the amount of rapidly labeled RNA released from nuclei in a reconstituted cell-free system. Cross-over experiments show that this increase is due to an enhanced transport capacity of the cytosol. The 60 kilodalton RNA transport factor is also present in the cytosol of tumor cells. Addition of the 60 kilodalton factor to normal liver cytosol causes the transport of repetitive RNA sequences similar to those transported from liver nuclei to tumor cell cytosol and those transported to the tumor cell cytoplasm in vivo. This factor modifies nuclear RNA restriction, at least in part, by eliciting the transport of repetitive RNA normally retained within the nucleus of the normal cell.
用肝癌致癌物3'-甲基-4-二甲基氨基偶氮苯（3'-MeDAB）处理大鼠会导致在肝细胞溶胶中出现一种60千道尔顿的癌胚蛋白。该因子在处理后40小时内出现，并与在重组无细胞系统中从细胞核释放的快速标记RNA量的增加相一致。交叉实验表明，这种增加是由于细胞溶胶转运能力增强所致。60千道尔顿的RNA转运因子也存在于肿瘤细胞的细胞溶胶中。将60千道尔顿的因子添加到正常肝细胞溶胶中会导致重复RNA序列的转运，类似于从肝细胞核转运到肿瘤细胞溶胶以及在体内转运到肿瘤细胞细胞质中的那些序列。该因子至少部分地通过引发正常保留在正常细胞核内的重复RNA的转运来改变核RNA限制。
