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源自大鼠小肠的上皮样细胞系的特性鉴定：细胞角蛋白丝的证实

Characterization of an epithelioid cell line derived from rat small intestine: demonstration of cytokeratin filaments.

作者信息

Blay J, Brown K D

出版信息

Cell Biol Int Rep. 1984 Jul;8(7):551-60. doi: 10.1016/0309-1651(84)90054-7.

DOI:10.1016/0309-1651(84)90054-7
PMID:6204784
Abstract

A cell line (RIE-1) of epithelioid morphology was established from rat small intestine. RIE-1 cells possess the normal rat diploid number of chromosomes (42) up to at least passage 20. Both early (less than 10) and late (greater than 20) passage cells are non-tumourigenic and do not form colonies in soft agarose. RIE-1 cells bind two epithelial-specific monoclonal antibodies. In addition, the cells exhibit a network of cytoskeletal filaments which are labelled by an antiserum to keratin. These findings provide strong evidence for an epithelial origin of the RIE-1 cell line.

摘要

从大鼠小肠建立了一种具有上皮样形态的细胞系（RIE-1）。RIE-1细胞在至少传代20次之前都具有正常大鼠的二倍体染色体数（42条）。早期传代（少于10代）和晚期传代（大于20代）的细胞均无致瘤性，且在软琼脂糖中不形成集落。RIE-1细胞可结合两种上皮特异性单克隆抗体。此外，这些细胞呈现出由角蛋白抗血清标记的细胞骨架丝网络。这些发现为RIE-1细胞系的上皮起源提供了有力证据。

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