Gurr J A, Kourides I A
Endocrinology. 1984 Aug;115(2):830-2. doi: 10.1210/endo-115-2-830.
We have studied the relationship between the levels of the alpha and beta subunit of TSH and alpha and TSH-beta mRNA content in pituitaries from euthyroid, short-term, and long-term hypothyroid mice and in various mouse TSH-secreting tumors. TSH, alpha, and TSH-beta were determined by specific radioimmunoassay. alpha and TSH-beta mRNA content was measured by dot blot hybridization with labeled alpha or TSH-beta plasmid probes. Normal pituitaries contained an excess of both alpha protein and alpha mRNA compared to beta. However, in hypothyroidism there was an increase in TSH and in particular in beta such that the alpha/beta protein ratio approached 1. Similarly, a marked increase in TSH-beta mRNA led to an alpha/TSH-beta mRNA ratio of approximately 1. Ratios of alpha/TSH-beta protein and mRNA in TSH-secreting tumors varied widely. There was a fair, but imperfect, correlation between alpha and TSH-beta protein and mRNA levels. Although transcriptional control of alpha and TSH-beta production appears likely, posttranscriptional regulatory mechanisms also seem to be involved.
我们研究了正常甲状腺小鼠、短期和长期甲状腺功能减退小鼠垂体以及各种小鼠促甲状腺激素（TSH）分泌肿瘤中TSH的α和β亚基水平与α和TSH-β mRNA含量之间的关系。通过特异性放射免疫测定法测定TSH、α和TSH-β。用标记的α或TSH-β质粒探针通过斑点印迹杂交法测量α和TSH-β mRNA含量。与β相比，正常垂体中α蛋白和α mRNA均过量。然而，在甲状腺功能减退症中，TSH增加，尤其是β增加，使得α/β蛋白比值接近1。同样，TSH-β mRNA的显著增加导致α/TSH-β mRNA比值约为1。TSH分泌肿瘤中α/TSH-β蛋白和mRNA的比值差异很大。α与TSH-β蛋白及mRNA水平之间存在一定但不完美的相关性。虽然α和TSH-β产生的转录控制似乎很可能，但转录后调节机制似乎也参与其中。
