Strong P N, Wood J N, Ivanyi J
Eur J Biochem. 1984 Jul 2;142(1):145-51. doi: 10.1111/j.1432-1033.1984.tb08262.x.
Hybridoma lines secreting monoclonal antibodies against a phospholipase-inactive derivative of the presynaptic neurotoxin, beta-bungarotoxin, have been established. These antibodies, either of the IgG1 or IgG2b isotype with affinities in the range 1-2 X 10(8) 1/mol, recognized a single immunodominant region of native beta-bungarotoxin, most probably located on the A (phospholipase homologue) chain of the toxin. Using plate-adsorbed radioimmunoassay procedures, antibodies reacted with native beta-bungarotoxin and other beta-bungarotoxin isotoxins as well as with the non-toxic phospholipase A also present in Bungarus multicinctus venom. Other phospholipase A enzymes and presynaptic phospholipase neurotoxins did not show any competition with beta-bungarotoxin in the radioimmunoassay. Globulin fractions of monoclonal antibodies partially inhibited the phospholipase activity of beta-bungarotoxin.
已建立分泌针对突触前神经毒素β-银环蛇毒素的磷脂酶无活性衍生物的单克隆抗体的杂交瘤细胞系。这些抗体为IgG1或IgG2b同种型，亲和力在1 - 2×10⁸ 1/mol范围内，识别天然β-银环蛇毒素的单个免疫显性区域，极有可能位于毒素的A（磷脂酶同源物）链上。使用平板吸附放射免疫测定程序，抗体与天然β-银环蛇毒素、其他β-银环蛇毒素同毒素以及多环眼镜蛇毒液中也存在的无毒磷脂酶A发生反应。在放射免疫测定中，其他磷脂酶A酶和突触前磷脂酶神经毒素与β-银环蛇毒素没有任何竞争。单克隆抗体的球蛋白组分部分抑制了β-银环蛇毒素的磷脂酶活性。
