犬脑脊液中免疫反应性血管紧张素作为脱天冬酰胺-1-血管紧张素II的特性研究。

Characterization of immunoreactive angiotensin in canine cerebrospinal fluid as Des-Asp1-angiotensin II.

Hutchinsón J S, Csicsmann J, Korner P I, Johnston C I

Clin Sci Mol Med. 1978 Feb;54(2):147-51. doi: 10.1042/cs0540147.

Abstract

Immunoreactive angiotensin II was measured in cerebrospinal fluid of four normal dogs. 2. The migration of this immunoreactive angiotensin II on polyacrylamide-slab gel electrophoresis was identical with the migration of the heptapeptide, Des-Asp1-angiotensin II, in each case. 3. The biological activity of the material from canine cerebrospinal fluid in a pressor bioassay was similar to that of Des-Asp1-angiotensin II. 4. The pressor activity of the canine material was abolished by treating the pressor bioassay rat with a competitive antagonistic analogue, Sar1-Ala8-angiotensin II. 5. The results suggest that the biologically active immunoreactive angiotension II present in normal canine cerebrospinal fluid is composed mainly of the heptapeptide fragment of angiotensin II, Des-Asp1-angiotensin II.

摘要

对四只正常犬的脑脊液中的免疫反应性血管紧张素II进行了测定。2. 在每种情况下，这种免疫反应性血管紧张素II在聚丙烯酰胺平板凝胶电泳上的迁移与七肽去天门冬氨酸1血管紧张素II的迁移相同。3. 犬脑脊液中物质在升压生物测定中的生物活性与去天门冬氨酸1血管紧张素II的生物活性相似。4. 用竞争性拮抗类似物Sar1-Ala8-血管紧张素II处理升压生物测定大鼠后，犬物质的升压活性被消除。5. 结果表明，正常犬脑脊液中存在的具有生物活性的免疫反应性血管紧张素II主要由血管紧张素II的七肽片段去天门冬氨酸1血管紧张素II组成。