一种使用经过鉴定的单克隆抗体针对人纤连蛋白的快速且高度灵敏的固相酶免疫测定法。

A rapid and highly sensitive solid-phase enzyme immunoassay specific for human fibronectin using a characterized monoclonal antibody.

作者信息

Salonen E M, Vartio T, Miggiano V, Stähli C, Tacács B, Virgallita G, De Petro G, Barlati S, Vaheri A

出版信息

J Immunol Methods. 1984 Aug 3;72(1):145-56. doi: 10.1016/0022-1759(84)90442-3.

PMID:6205094

Abstract

A solid-phase enzyme immunoassay (EIA) was developed for the quantitation of human fibronectin in body fluids and cell culture media. In the assay a human fibronectin-specific murine monoclonal IgG1 (f-33) was used as capture antibody and polyclonal rabbit anti-fibronectin as detector antibody. The antibody showed no reactivity to purified monkey, dog, rabbit, horse, sheep, mouse, bovine or chicken fibronectins. The determinant of the monoclonal antibody was mapped to the cell-binding region of the fibronectin molecule. This localization was based on the use of purified fragments of fibronectin, immunoblotting, EIA and inhibition of fibroblast adhesion and spreading by the antibody. The detection limit of the fibronectin assay was 2 ng/ml. The assay was used for the quantitation of fibronectin in human plasma, urine and cerebrospinal fluid specimens and culture media of human cells.

摘要

已开发出一种固相酶免疫测定法（EIA），用于定量检测体液和细胞培养基中的人纤连蛋白。在该测定法中，使用人纤连蛋白特异性鼠单克隆IgG1（f - 33）作为捕获抗体，并用兔抗纤连蛋白多克隆抗体作为检测抗体。该抗体对纯化的猴、狗、兔、马、羊、小鼠、牛或鸡纤连蛋白无反应性。单克隆抗体的决定簇定位于纤连蛋白分子的细胞结合区域。这种定位是基于使用纯化的纤连蛋白片段、免疫印迹、EIA以及该抗体对成纤维细胞粘附和铺展的抑制作用。纤连蛋白测定法的检测限为2 ng/ml。该测定法用于定量检测人血浆、尿液、脑脊液标本以及人细胞培养基中的纤连蛋白。