Glant T T, Hadházy C, Mikecz K, Sipos A
Histochemistry. 1985;82(2):149-58. doi: 10.1007/BF00708199.
Binding of fibronectins (FN) to collagen types I-IV were studied using polyclonal antibodies against human and chicken FNs, proteoglycan monomers, collagen type II and monoclonal antibodies reacting with both soluble and insoluble forms of human FN. Plasma fibronectin and type II collagen were shown to interact specifically in a homologous system. Type II collagen, however, proved to be less effective in inhibition assays compared to other types of collagen. In high density cultures of chicken limb bud cells, fibronectin was first localized within the fibroblast-like cells of 4 hr cultures and an extensive extracellular filamentous network developed by the end of day 1. Fibronectin was present in the newly formed cartilage nodules although it seemed to disappear by day 6, when the proteoglycan accumulation became more intensive. Enzyme treatments (testicular hyaluronidase, chondroitinase ABC) helped to localize FN at this stage of development of chicken cartilage, in microdroplet high density cultures of human fetal chondrocytes and in articular cartilage. Fibronectin was localized only in the pericellular ring of intact human articular cartilage using monoclonal antibodies with the biotin-avidin system.
利用抗人及鸡纤连蛋白（FN）、蛋白聚糖单体、II型胶原的多克隆抗体以及与可溶性和不溶性人FN均反应的单克隆抗体，研究了纤连蛋白（FN）与I - IV型胶原的结合。血浆纤连蛋白和II型胶原在同源系统中显示出特异性相互作用。然而，与其他类型的胶原相比，II型胶原在抑制试验中效果较差。在鸡肢芽细胞的高密度培养中，纤连蛋白最初定位于4小时培养物中的成纤维细胞样细胞内，到第1天结束时形成广泛的细胞外丝状网络。纤连蛋白存在于新形成的软骨结节中，不过在第6天似乎消失了，此时蛋白聚糖积累更为密集。酶处理（睾丸透明质酸酶、软骨素酶ABC）有助于在鸡软骨发育的这个阶段、人胎儿软骨细胞的微滴高密度培养以及关节软骨中定位FN。使用生物素 - 抗生物素蛋白系统的单克隆抗体，纤连蛋白仅定位于完整人关节软骨的细胞周围环中。
