Abraham G, Yarom R, Manor H
Virology. 1984 Jul 30;136(2):442-7. doi: 10.1016/0042-6822(84)90181-8.
Polyadenylated transcripts of a monkey lymphotropic papovavirus (LPV), which can be propagated in monkey and human lymphoblastoid cell lines, were identified and mapped. Polyadenylated RNA was purified from cells of the human line BJA/B infected with LPV, and was hybridized with various LPV DNA fragments. The hybrids were treated with S1 endonuclease and analyzed by alkaline gel electrophoresis. This analysis revealed two groups of RNA molecules encoded in two regions of the LPV genome. One group includes four colinear transcripts of 2.3, 2.1, 1.85, and 1.2 kb, whose polyadenylated termini map at a single site on the LPV DNA. The second group includes two less-abundant transcripts of 1.8 and 1.95 kb. The polarities of the LPV transcripts were determined by hybridizing cDNA complementary to their 3' termini with LPV DNA fragments. The two groups were found to have opposite polarities. It is shown that the four major transcripts can be aligned with, and may be functionally related to, SV40 and polyoma virus "late" mRNAs. It is also inferred that the 1.8- and 1.95-kb RNA species may be functionally related to the "early" transcripts of these papovaviruses.
一种可在猴和人淋巴母细胞系中增殖的猴嗜淋巴细胞乳头瘤病毒（LPV）的多聚腺苷酸化转录本被鉴定并进行了图谱分析。从感染LPV的人BJA/B细胞系的细胞中纯化出多聚腺苷酸化RNA，并使其与各种LPV DNA片段杂交。杂交体用S1核酸酶处理，并通过碱性凝胶电泳进行分析。该分析揭示了在LPV基因组的两个区域编码的两组RNA分子。一组包括2.3、2.1、1.85和1.2 kb的四个共线性转录本，其多聚腺苷酸化末端定位在LPV DNA的单个位点上。第二组包括1.8和1.95 kb的两个丰度较低的转录本。通过将与其3'末端互补的cDNA与LPV DNA片段杂交来确定LPV转录本的极性。发现这两组具有相反的极性。结果表明，这四个主要转录本可以与SV40和多瘤病毒的“晚期”mRNA排列在一起，并且可能在功能上相关。还推断1.8和1.95 kb的RNA种类可能在功能上与这些乳头瘤病毒的“早期”转录本相关。
