Tamamaki N, Watanabe K, Nojyo Y
Brain Res. 1984 Jul 30;307(1-2):336-40. doi: 10.1016/0006-8993(84)90489-x.
The intracellular pressure-injection of HRP was applied to the rat hippocampus and has brought an excellent presentation of the Golgi-like image of the pyramidal neuron. Rats were allowed to survive for 3 days and brain sections were treated with the PAP-immunohistochemical technique to enhance the sensitivity of HRP neurohistochemistry. The pyramidal neuron densely developed axon branches in the ipsilateral hippocampus and sent the commissural axon to the contralateral hippocampus. Moreover, short axon branches diverged from the commissural axon to the bilateral septal nuclei.
将辣根过氧化物酶（HRP）进行细胞内压力注射到大鼠海马体中，呈现出了极佳的锥体细胞高尔基样图像。让大鼠存活3天，然后用PAP免疫组织化学技术处理脑切片，以提高HRP神经组织化学的敏感性。锥体细胞在同侧海马体中密集地发出轴突分支，并将连合轴突发送到对侧海马体。此外，短轴突分支从连合轴突发散到双侧隔核。
