Anthony A, Doebler J A, Bocan T M, Zerweck C, Shih T M
Cell Biochem Funct. 1983 Apr;1(1):30-6. doi: 10.1002/cbf.290010106.
Cytophotometric analyses of RNA and acetylcholinesterase responses of caudate and cerebrocortical neurons of soman toxicated rats were conducted to characterize impairments in regulatory aspects of neuronal metabolism occurring in the acute phase of cholinesterase impairment. There was a severe and dose-dependent suppression (20-60%) in neuronal acetylcholinesterase activity in both a.m. and p.m.-treated rats; no diurnal differences were apparent in control acetylcholinesterase levels or neuronal acetylcholinesterase responsiveness to soman toxication. RNA levels, however, were markedly higher in p.m. than in a.m. saline-treated controls. Soman depressed caudate neuron RNA contents in the afternoon, but not in the morning. Cerebrocortical neuron RNA levels were suppressed in both a.m. and p.m.-toxicated rats, although this RNA depletion was more severe in the afternoon. These results indicate that soman can elicit marked alterations in neuronal transcriptional-translational capabilities and that there are diurnal variations in cellular metabolic responsiveness to soman toxication. Although functional relationships between soman-induced cholinesterase inhibition and RNA depletion remain to be elucidated, depressed RNA metabolism appears to be a maladaptive response preventing rapid regeneration of cholinesterase following poisoning.
对梭曼中毒大鼠的尾状核和大脑皮质神经元的RNA及乙酰胆碱酯酶反应进行了细胞光度分析，以表征在胆碱酯酶损伤急性期发生的神经元代谢调节方面的损伤。上午和下午给药的大鼠神经元乙酰胆碱酯酶活性均出现严重且剂量依赖性的抑制（20%-60%）；对照乙酰胆碱酯酶水平或神经元乙酰胆碱酯酶对梭曼中毒的反应性没有明显的昼夜差异。然而，下午给药的大鼠RNA水平明显高于上午给予生理盐水的对照大鼠。梭曼在下午降低了尾状核神经元的RNA含量，但在上午没有。上午和下午中毒的大鼠大脑皮质神经元RNA水平均受到抑制，尽管下午这种RNA耗竭更为严重。这些结果表明，梭曼可引起神经元转录-翻译能力的显著改变，并且细胞对梭曼中毒的代谢反应存在昼夜变化。尽管梭曼诱导的胆碱酯酶抑制与RNA耗竭之间的功能关系仍有待阐明，但RNA代谢降低似乎是一种适应不良的反应，可阻止中毒后胆碱酯酶的快速再生。
