识别中间丝蛋白、肌动蛋白和肌球蛋白上共同抗原决定簇的单克隆抗体。

Monoclonal antibody which recognizes a common antigenic determinant on intermediate filament proteins, actin, and myosin.

作者信息

Franklin R M, Emmons L R, Emmons R P, Oommen A, Pink J R, Rijnbeek A M, Schnetzler M, Tuderman L, Vainio E

出版信息

Hybridoma. 1983;2(3):275-85. doi: 10.1089/hyb.1983.2.275.

DOI:10.1089/hyb.1983.2.275

PMID:6205979

Abstract

A monoclonal antibody is described which reacts with the intermediate filament proteins vimentin, desmin, keratins, actin, and myosin. This is the first report of an epitope common to intermediate filament proteins and myosin. X1, the wide-spectrum monoclonal antibody in question, was isolated in the course of screening monoclonal antibodies to chicken thymocytes. Cross-reactivities were investigated by immunofluorescence on various types of cultured cells and sectioned tissues, ELISA with a panel of purified antigens, immunoprecipitation, immunodot tests, and immunoblotting.

摘要

描述了一种单克隆抗体，它可与中间丝蛋白波形蛋白、结蛋白、角蛋白、肌动蛋白和肌球蛋白发生反应。这是关于中间丝蛋白和肌球蛋白共有表位的首次报道。所讨论的广谱单克隆抗体X1是在筛选针对鸡胸腺细胞的单克隆抗体过程中分离得到的。通过对各种类型的培养细胞和切片组织进行免疫荧光、用一组纯化抗原进行酶联免疫吸附测定、免疫沉淀、免疫斑点试验和免疫印迹来研究交叉反应性。