Lei H Y, Waltenbaugh C
J Immunol. 1984 Oct;133(4):1723-9.
A/J, A/WySn (H-2a, I-Jk), and B6 (H-2b, I-Jb) mice are poly(Glu50Tyr50)(GT) nonresponders. GT preimmunization does not suppress humoral immune responses to the immunogenic form of GT, GT-MBSA (GT-methylated bovine serum albumin), in these strains. B6 mice produce an idiotypic GT-specific T cell-derived suppressor factor (GT-TsF1) after GT injection, but cannot be suppressed by this or any other GT-TsF1. H-2a mice do not produce GT-TsF1, but can be suppressed by B6 GT-TsF1. Injection of cellfree B6 GT-TsF1 into H-2a mice leads to the production of two distinct I-Jk GT-TsF. We have termed these GT-TsF2 and GT-TsF3 on the basis of their kinetic activity and antigenic markers. Anti-idiotypic I-Jk GT-TsF2 is suppressive at a time intermediate between GT-TsF1 and GT-TsF3. Idiotypic I-Jk GT-TsF3 suppresses GT-MBSA PFC responses the day of PFC assay. GT-TsF3, not GT-TsF1, suppresses B6 GT-MBSA PFC responses, indicating that these idiotypic factors are distinct. Our results establish that H-2a lack or have a defective Ts1 cell for GT suppression, and H-2b mice lack a functional Ts2 cell. These findings offer strategies for circumventing suppressor T cell defects in "nonsuppressors."
A/J、A/WySn（H-2a，I-Jk）和B6（H-2b，I-Jb）小鼠是聚（Glu50Tyr50）（GT）无反应者。在这些品系中，GT预免疫不会抑制对免疫原性形式的GT即GT-MBSA（GT-甲基化牛血清白蛋白）的体液免疫反应。B6小鼠在注射GT后会产生一种独特型GT特异性T细胞衍生抑制因子（GT-TsF1），但不能被这种或任何其他GT-TsF1所抑制。H-2a小鼠不产生GT-TsF1，但可被B6 GT-TsF1所抑制。将无细胞的B6 GT-TsF1注射到H-2a小鼠体内会导致产生两种不同的I-Jk GT-TsF。基于它们的动力学活性和抗原标记，我们将这些GT-TsF分别命名为GT-TsF2和GT-TsF3。抗独特型I-Jk GT-TsF2在GT-TsF1和GT-TsF3之间的中间时间具有抑制作用。独特型I-Jk GT-TsF3在PFC检测当天抑制GT-MBSA PFC反应。是GT-TsF3而非GT-TsF1抑制B6 GT-MBSA PFC反应，这表明这些独特型因子是不同的。我们的结果表明，H-2a小鼠缺乏或具有有缺陷的用于GT抑制的Ts1细胞，而H-2b小鼠缺乏功能性的Ts2细胞。这些发现为规避“非抑制者”中抑制性T细胞缺陷提供了策略。
