Sorensen C M, Pierce C W
J Immunol. 1985 Jul;135(1):362-7.
Fusion of spleen cells from rats hyperimmunized with T cell hybridoma derived GAT-specific TsF1 or TsF2 suppressor T cell factors has resulted in the generation of hybridomas secreting monoclonal antibodies reactive with the appropriate GAT-TsF used for immunization, and in several cases, reactive with other GAT-TsF1 and TsF2. The monoclonal anti-TsF1 antibodies are capable of modulating in vitro GAT-specific PFC response in a GAT-specific manner; some suppress responses to GAT directly, whereas others reverse GAT-TsF1-mediated suppression of responses. The monoclonal anti-TsF2 antibodies all reverse suppression but are reactive with combinatorial determinants, I-J+ chains or antigen-binding chains of the GAT-TsF2. The data are discussed in terms of the nature of the determinants recognized by these antibodies as well as the potential uses of these reagents for studying the suppressor T cell pathway and potential relationships between Ts1, Ts2, and T helper cells.
用源自T细胞杂交瘤的GAT特异性TsF1或TsF2抑制性T细胞因子对大鼠进行超免疫后，将其脾细胞进行融合，结果产生了分泌单克隆抗体的杂交瘤，这些单克隆抗体与用于免疫的相应GAT - TsF反应，在某些情况下，还与其他GAT - TsF1和TsF2反应。单克隆抗TsF1抗体能够以GAT特异性方式在体外调节GAT特异性PFC反应；一些直接抑制对GAT的反应，而另一些则逆转GAT - TsF1介导的反应抑制。单克隆抗TsF2抗体均能逆转抑制作用，但与GAT - TsF2的组合决定簇、I - J +链或抗原结合链反应。本文根据这些抗体识别的决定簇的性质以及这些试剂在研究抑制性T细胞途径以及Ts1、Ts2和辅助性T细胞之间潜在关系方面的潜在用途对数据进行了讨论。
