Nathan C F, Prendergast T J, Wiebe M E, Stanley E R, Platzer E, Remold H G, Welte K, Rubin B Y, Murray H W
J Exp Med. 1984 Aug 1;160(2):600-5. doi: 10.1084/jem.160.2.600.
Cytokines affecting mononuclear phagocytes were screened for activation of human macrophages to secrete H2O2 and kill toxoplasmas. In contrast to recombinant interferon-gamma (rIFN gamma), the following factors, tested in partially or highly purified form and over a wide range of concentrations, did not augment these functions: native interferon-alpha (nIFN alpha), rIFN alpha A, rIFN alpha D, rIFN beta, colony stimulating factor (type 1) (CSF-1), CSF for granulocytes and macrophages (GM-CSF), pluripotent CSF (p-CSF), tumor necrosis factor (TNF), native interleukin 2 (nIL-2), and rIL-2. Partially purified migration inhibitory factor (MIF) enhanced H2O2-releasing capacity submaximally without inducing antitoxoplasma activity, and warrants further study.
筛选影响单核吞噬细胞的细胞因子,以激活人类巨噬细胞分泌过氧化氢并杀死弓形虫。与重组干扰素-γ(rIFNγ)相反,以下以部分纯化或高度纯化形式在广泛浓度范围内测试的因子并未增强这些功能:天然干扰素-α(nIFNα)、rIFNαA、rIFNαD、rIFNβ、集落刺激因子(1型)(CSF-1)、粒细胞和巨噬细胞集落刺激因子(GM-CSF)、多能集落刺激因子(p-CSF)、肿瘤坏死因子(TNF)、天然白细胞介素2(nIL-2)和rIL-2。部分纯化的迁移抑制因子(MIF)在未诱导抗弓形虫活性的情况下,最大程度地增强了过氧化氢释放能力,值得进一步研究。