Kimura H, Pickard A, Wilson D B
J Exp Med. 1984 Sep 1;160(3):652-8. doi: 10.1084/jem.160.3.652.
F1 hybrid (A X B) rats immunized with parental strain (A) T cells, or which recover from graft-versus-host (GVH) reactions caused by parental T cells, develop strong T cell-mediated immune responses against anti-major histocompatibility complex (MHC) receptor structures on donor T cells specific for host (MHCb) alloantigens. This immune response provides the basis for a profound and specific resistance to the subsequent induction in these animals of local or systemic GVH disease. Using subset-specific monoclonal antibodies and negative selection rosetting procedures, we attempted to determine which donor T cell subset possesses the immunogenic idiotypic markers, and which host T cell subpopulation mediates GVH resistance. We show here that the immunizing donor cell population belongs to the W3/25+ helper T cell (Th) subset, the same one that causes local GVH reactions, and that both the W3/25+ Th and the OX8+ killer/suppressor (Tk/s) subsets of host T cells are able to transfer GVH resistance to secondary F1 recipients.
用亲代品系(A)T细胞免疫的F1杂种(A×B)大鼠,或从亲代T细胞引起的移植物抗宿主(GVH)反应中恢复的大鼠,会针对宿主(MHCb)同种异体抗原特异性的供体T细胞上的抗主要组织相容性复合体(MHC)受体结构产生强烈的T细胞介导的免疫反应。这种免疫反应为这些动物对随后诱导的局部或全身性GVH疾病产生深刻而特异性的抗性提供了基础。使用亚群特异性单克隆抗体和阴性选择玫瑰花结程序,我们试图确定哪些供体T细胞亚群具有免疫原性独特型标记,以及哪些宿主T细胞亚群介导GVH抗性。我们在此表明,免疫供体细胞群体属于W3/25+辅助性T细胞(Th)亚群,即引起局部GVH反应的同一亚群,并且宿主T细胞的W3/25+Th和OX8+杀伤/抑制(Tk/s)亚群都能够将GVH抗性转移给二次F1受体。