Deming J W, Hada H, Colwell R R, Luehrsen K R, Fox G E
J Gen Microbiol. 1984 Aug;130(8):1911-20. doi: 10.1099/00221287-130-8-1911.
Deep-sea bacteria were isolated from the digestive tract of animals inhabiting depths of 5900 m in the Puerto Rico Trench and 4300 m near the Walvis Ridge. Growth of two bacterial strains was measured in marine broth and in solid media under a range of pressures and temperatures. Both strains were barophilic at 2 degrees C (+/- 1 degrees C) with an optimal growth rate of 0.22 h-1 at a pressure 30% lower than that encountered in situ. At 1 atm they grew at temperatures ranging from 1.2 to 18.2 degrees C (+/- 0.3 degrees C), while in situ pressures increased the upper temperature limit to 23.3 degrees C. Both strains were identified as members of the genus Vibrio, based on standard taxonomic tests and mol% G + C values (47.0 and 47.1). Ribonucleotide sequences determined for 5S ribosomal RNA from each strain confirmed relationship to the Vibrio-Photobacterium group, as represented by V. harveyi and P. phosphoreum, but the barophiles were clearly distinct from these species. Secondary structure conformed to the established model for eubacterial 5S rRNA.
深海细菌是从栖息在波多黎各海沟5900米深处以及靠近瓦尔维斯脊4300米处的动物消化道中分离出来的。在一系列压力和温度条件下，测定了两种细菌菌株在海洋肉汤和固体培养基中的生长情况。两种菌株在2℃（±1℃）时为嗜压菌，在比原位压力低30%的压力下，最佳生长速率为0.22 h-1。在1个大气压下，它们在1.2至18.2℃（±0.3℃）的温度范围内生长，而原位压力将温度上限提高到23.3℃。根据标准分类测试和mol% G + C值（47.0和47.1），两种菌株均被鉴定为弧菌属成员。对每个菌株的5S核糖体RNA测定的核糖核苷酸序列证实了它们与以哈维氏弧菌和发光杆菌为代表的弧菌-发光杆菌菌群的关系，但嗜压菌与这些物种明显不同。二级结构符合已建立的真细菌5S rRNA模型。
