Kamalakannan V, Sathyamoorthy A V, Motlag D B
Res Exp Med (Berl). 1984;184(2):91-101. doi: 10.1007/BF01852356.
The effect of raw black gram and black gram trypsin inhibitory fraction on the pancreatic function of male albino mice has been evaluated. Animals were fed autoclaved black gram (Group I), raw black gram (Group II) and autoclaved black gram incorporated with black gram trypsin inhibitory fraction (Group III). Pancreatic total protease, trypsin and amylase activities were elevated significantly in Group II and III animals. Analyses of intestinal contents showed a constant level of total protease activity in all the groups, along with a significant reduction in trypsin level in Groups II and III. Increased U-14C-lysine incorporation in pancreas and an enhanced level of amylase in intestinal contents show the hyperactivity of the pancreas in Groups II and III. Pancreas weights increased in Groups II and III. Pancreatic protein/mg pancreas and RNA/DNA ratio remained constant in all three groups. It is concluded with histological studies that the black gram trypsin inhibitor increased the pancreatic activity of mice by developing the cellular hyperplasia of the pancreas.
已评估生黑绿豆和黑绿豆胰蛋白酶抑制组分对雄性白化小鼠胰腺功能的影响。给动物喂食高压灭菌的黑绿豆（第一组）、生黑绿豆（第二组）以及添加了黑绿豆胰蛋白酶抑制组分的高压灭菌黑绿豆（第三组）。第二组和第三组动物的胰腺总蛋白酶、胰蛋白酶和淀粉酶活性显著升高。对肠道内容物的分析表明，所有组的总蛋白酶活性水平保持恒定，而第二组和第三组的胰蛋白酶水平显著降低。第二组和第三组胰腺中U-14C-赖氨酸掺入增加以及肠道内容物中淀粉酶水平升高表明胰腺功能亢进。第二组和第三组的胰腺重量增加。三组的胰腺蛋白质/毫克胰腺和RNA/DNA比率保持恒定。组织学研究得出结论，黑绿豆胰蛋白酶抑制剂通过促进胰腺细胞增生增加了小鼠的胰腺活性。
