Maxwell M H
Res Vet Sci. 1984 Jul;37(1):7-11.
A staining technique was developed for the differential identification of tissue eosinophil and heterophil leucocytes in the fowl. Pieces of formalin-fixed skin, challenged with dinitrochlorobenzene (DNCB) or citraconic anhydride (CA), were incubated in a substrate suitable for peroxidase prior to embedding in either paraffin wax, glycol methacrylate or Araldite. This results in deep brown staining of the eosinophil granules while those of the heterophils remain unstained. Heterophils and eosinophils were conspicuous at 30 minutes after challenge in the early inflammatory response. By 48 hours the heterophilic response had diminished and eosinophils had almost disappeared. Only mononuclear cells were seen at 72 hours. It is suggested that the eosinophil leucocyte might act as an early modulator of inflammation in delayed-type hypersensitivity responses in the fowl.
开发了一种染色技术，用于鉴别禽类组织中的嗜酸性粒细胞和嗜异性白细胞。将经二硝基氯苯（DNCB）或柠康酸酐（CA）激发的福尔马林固定皮肤切片，在包埋于石蜡、甲基丙烯酸乙二醇酯或环氧树脂之前，在适合过氧化物酶的底物中孵育。这导致嗜酸性粒细胞颗粒呈深棕色染色，而嗜异性粒细胞颗粒则保持未染色。在激发后的早期炎症反应中，30分钟时嗜异性粒细胞和嗜酸性粒细胞很明显。到48小时时，嗜异性反应减弱，嗜酸性粒细胞几乎消失。72小时时仅可见单核细胞。提示嗜酸性粒细胞可能在禽类迟发型超敏反应中作为炎症的早期调节因子发挥作用。
