Cheresh D A, Reisfeld R A, Varki A P
Science. 1984 Aug 24;225(4664):844-6. doi: 10.1126/science.6206564.
Monoclonal antibody Mab D1.1 recognizes on human melanoma cells a ganglioside antigen characterized by an alkali-labile O-acetylated sialic acid residue. Immunochemical analysis showed that this molecule is an O-acetylated product of the neuroectoderm-associated disialoganglioside GD3. Controlled chemical O-acetylation of purified GD3 resulted in the generation of this same epitope. Lysates of human melanoma cells were found to contain O-acetyltransferase activity capable of generating the antigenic epitope recognized by Mab D1.1. Thus, the addition of a single O-acetyl group to a common cell surface-associated ganglioside can create a potentially tumor-specific antigen.
单克隆抗体Mab D1.1在人黑色素瘤细胞上识别一种神经节苷脂抗原，其特征为含有一个对碱不稳定的O-乙酰化唾液酸残基。免疫化学分析表明，该分子是神经外胚层相关的二唾液酸神经节苷脂GD3的O-乙酰化产物。纯化的GD3经可控化学O-乙酰化后产生了相同的表位。发现人黑色素瘤细胞裂解物含有能够产生被Mab D1.1识别的抗原表位的O-乙酰转移酶活性。因此，在常见的细胞表面相关神经节苷脂上添加单个O-乙酰基团可产生一种潜在的肿瘤特异性抗原。
