使用一种新的银浸染技术对垂体中间部星状细胞的显示。

Demonstration of stellate cells of the pars intermedia of the pituitary gland using a new silver impregnation technique.

作者信息

de Bold M L, de Bold A J, Kraicer J

出版信息

Stain Technol. 1984 Jan;59(1):49-52. doi: 10.3109/10520298409113828.

DOI:10.3109/10520298409113828

PMID:6206623

Abstract

The pars intermedia of the pituitary gland in the rat and other species contains, in addition to secretory cells, stellate cells first characterized by using electron microscopy. The distribution and relationships of these cells is difficult to assess at the ultrastructural level. We have developed an ammoniacal silver nitrate method for stellate cells of the pars intermedia. Staining is carried out in 15 micron sections of buffered formalin fixed, paraffin embedded pituitaries. This method shows that in the rat pars intermedia, stellate cells showing numerous cytoplasmic projections are abundant in, and evenly distributed throughout, pars intermedia lobules.

摘要

大鼠及其他物种垂体中间部除分泌细胞外，还含有最早通过电子显微镜鉴定的星形细胞。在超微结构水平上，这些细胞的分布和关系难以评估。我们开发了一种用于垂体中间部星形细胞的氨性硝酸银法。染色在经缓冲福尔马林固定、石蜡包埋的垂体15微米切片上进行。该方法显示，在大鼠垂体中间部，具有大量细胞质突起的星形细胞在中间部小叶中丰富且均匀分布。