Mouches C, Candresse T, McGarrity G J, Bové J M
Yale J Biol Med. 1983 Sep-Dec;56(5-6):431-7.
Spiroplasma strains of group IV were compared by two-dimensional protein analyses on polyacrylamide gels. Although considerable diversity was evident, the assemblages studied were less heterogeneous than the known strains of group I. Two electrophoretic techniques were used to identify spiroplasma proteins that had been used to immunize rabbits. These included monoclonal antibodies prepared against Spiroplasma citri. In the first technique, protein antigens were purified by immunoaffinity chromatography, then identified with SDS-PAGE. In the second technique, spiroplasma proteins were first separated by SDS-PAGE, then antigens were identified by antibody binding to blot-transferred proteins. Finally, two-dimensional protein electrophoresis has been used as a source of immunogens to characterize monospecific antibodies against individual S. citri proteins.
通过聚丙烯酰胺凝胶上的二维蛋白质分析对IV组的螺原体菌株进行了比较。尽管明显存在相当大的多样性，但所研究的群体比已知的I组菌株的异质性要小。使用了两种电泳技术来鉴定已用于免疫兔子的螺原体蛋白质。这些包括针对柑橘螺原体制备的单克隆抗体。在第一种技术中，蛋白质抗原通过免疫亲和色谱法纯化，然后用SDS-PAGE进行鉴定。在第二种技术中，螺原体蛋白质首先通过SDS-PAGE分离，然后通过抗体与印迹转移蛋白的结合来鉴定抗原。最后，二维蛋白质电泳已被用作免疫原的来源，以表征针对单个柑橘螺原体蛋白质的单特异性抗体。
