Mouches C, Bové J M
Yale J Biol Med. 1983 Sep-Dec;56(5-6):723-7.
A plasmid, pM41, has been isolated from the Spiroplasma citri strain M4 (group I-1) and characterized by restriction mapping. Using a 32P-labeled probe specific of the plasmid, we have shown by DNA-DNA hybridization that a plasmid identical to pM41 or a closely related plasmid, is present in several, but not all, S. citri strains. DNA sequences that hybridize to pM41 were also identified in three other spiroplasmas not belonging to the S. citri species. Protein patterns of several S. citri strains have been compared in order to investigate the effect of pM41 on the spiroplasma protein profiles or maps. In fact, the presence of pM41 does not appear to modify the protein pattern.
从柑橘螺原体菌株M4（I-1组）中分离出了一种质粒pM41，并通过限制性酶切图谱对其进行了表征。使用针对该质粒的32P标记探针，我们通过DNA-DNA杂交表明，在若干但并非所有的柑橘螺原体菌株中都存在与pM41相同或密切相关的质粒。在另外三种不属于柑橘螺原体种的螺旋体中也鉴定出了与pM41杂交的DNA序列。为了研究pM41对螺原体蛋白质谱或图谱的影响，对几种柑橘螺原体菌株的蛋白质模式进行了比较。事实上，pM41的存在似乎并未改变蛋白质模式。
