由一种链霉菌产生的脑谷氨酸脱羧酶抑制剂4,5-二羟基间苯二甲酸的分离。

Isolation of 4.5-dihydroxyisophthalic acid an inhibitor of brain glutamate decarboxylase, produced by a Streptomyces species.

作者信息

Endo A, Kitahara N, Oka H, Miguchi-Fukazawa Y, Terahara A

出版信息

Eur J Biochem. 1978 Jan 2;82(1):257-9. doi: 10.1111/j.1432-1033.1978.tb12018.x.

DOI:10.1111/j.1432-1033.1978.tb12018.x

PMID:620673

Abstract

A potent inhibitor of brain glutamate decarboxylase (L-glutamate 1-carboxy-lyase, EC 4.1.1.15) was isolated from cultures of a Streptomyces species, and its structure was found to be 4,5-dihydroxyisophthalic acid. The metabolite inhibited beef brain glutamate decarboxylase 50% at a concentration of 0.12 microgram/ml (0.61 micron). The kinetic analysis indicated that 4,5-dihydroxyisophthalic acid was a competitive inhibitor having a Ki value of 0.18 micron.

摘要

从一种链霉菌属菌株的培养物中分离出一种强效的脑谷氨酸脱羧酶（L-谷氨酸1-羧基裂解酶，EC 4.1.1.15）抑制剂，其结构被确定为4,5-二羟基间苯二甲酸。该代谢产物在浓度为0.12微克/毫升（0.61微摩尔）时可抑制牛脑谷氨酸脱羧酶50%。动力学分析表明，4,5-二羟基间苯二甲酸是一种竞争性抑制剂，其Ki值为0.18微摩尔。