Marshall J H, Bridge P D, May J W
Anal Biochem. 1984 Jun;139(2):359-62. doi: 10.1016/0003-2697(84)90017-4.
During a study of the distribution of several NAD-linked dehydrogenase enzymes in various yeasts, in which polyacrylamide gel electrophoresis, followed by activity staining with phenazine methosulfate and a tetrazolium was used, a band was frequently detected, the production of which appeared to be independent of any added substrate (the "nothing dehydrogenase" effect). It has been shown that this effect is caused by alcohol dehydrogenase acting on traces of ethanol inadvertently introduced into the system. Two sources of ethanol were identified. They were (i) the enzyme extracts, which could be freed from ethanol by gel filtration, and (ii) the acrylamide used to prepare the gel, which could be freed from ethanol by recrystallization from ethanol-free chloroform. It is suggested that the use of commercial chloroform (stabilized with ethanol) as a recrystallizing solvent is the source of ethanol contamination in commercial preparations of acrylamide.
在一项对多种酵母中几种与NAD相关的脱氢酶分布的研究中，使用了聚丙烯酰胺凝胶电泳，随后用硫酸吩嗪和四氮唑进行活性染色，经常检测到一条带，其产生似乎与任何添加的底物无关（“无底物脱氢酶”效应）。研究表明，这种效应是由酒精脱氢酶作用于系统中无意中引入的微量乙醇引起的。确定了乙醇的两个来源。它们是：（i）酶提取物，可通过凝胶过滤除去乙醇；（ii）用于制备凝胶的丙烯酰胺，可通过从无乙醇氯仿中重结晶除去乙醇。有人认为，使用用乙醇稳定的市售氯仿作为重结晶溶剂是丙烯酰胺商业制剂中乙醇污染的来源。
