Ziboh V A, Isseroff R R, Pandey R
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1234-40. doi: 10.1016/0006-291x(84)91224-5.
The present findings associate phospholipid alteration, particularly the turnover of phosphatidylinositol, in Ca2+ induced differentiation of keratinocytes. These conclusions are based on the hydrolysis of 14C-AA from prelabeled PI and the accumulation 14C-DG and 14C-PA after cells are switched from low to normal concentrations of extracellular Ca2+. This novel finding implies that the biological changes which accompany keratinocyte differentiation after switch from low to normal extracellular medium may be due at least in part to increased accumulation of PA and DG which are major deacylation and reacylation products of phosphatidylinositol. A second interesting finding in these studies is the marked transformation of 14C-AA into lipoxygenase products by proliferating keratinocytes cultured in low Ca2+ medium when compared to differentiating cells cultured in normal Ca2+. The significance of decreased generation of lipoxygenase products in epidermal differentiation deserve further exploration.
目前的研究结果表明，磷脂变化，尤其是磷脂酰肌醇的周转，与钙离子诱导的角质形成细胞分化有关。这些结论基于以下观察：在细胞从低细胞外钙离子浓度转换到正常浓度后，预先标记的磷脂酰肌醇中14C-花生四烯酸的水解，以及14C-二酰甘油和14C-磷脂酸的积累。这一新颖的发现表明，在从低细胞外培养基转换到正常培养基后，伴随角质形成细胞分化的生物学变化可能至少部分归因于磷脂酸和二酰甘油积累的增加，而它们是磷脂酰肌醇的主要去酰化和再酰化产物。这些研究中的另一个有趣发现是，与在正常钙离子浓度下培养的分化细胞相比，在低钙离子培养基中培养的增殖角质形成细胞将14C-花生四烯酸显著转化为脂氧合酶产物。表皮分化过程中脂氧合酶产物生成减少的意义值得进一步探索。
