Leong J C, Nelson J A, Levy J A
Biochim Biophys Acta. 1984 Sep 10;782(4):441-5. doi: 10.1016/0167-4781(84)90052-6.
Optimal conditions for detecting reverse transcriptase activity in human placental extracts are described. They vary with the state of the placenta at birth and are influenced by relative amounts of detergent, monovalent cation, and protein in the reaction mixture. Demonstrating activity of the placental enzyme requires detergent, but the enzyme is sensitive to high detergent concentrations. This sensitivity can be be altered by lowering the monovalent cation concentration from 0.154 to 0.034 M and by adding protein to the reaction mixture. The detection of reverse transcriptase in Rauscher murine leukemia virus and baboon endogenous type C virus, but not in the Mason-Pfizer monkey type D virus, shows similar requirements.
本文描述了检测人胎盘提取物中逆转录酶活性的最佳条件。这些条件随出生时胎盘的状态而变化，并受反应混合物中去污剂、单价阳离子和蛋白质相对含量的影响。证明胎盘酶的活性需要去污剂，但该酶对高浓度去污剂敏感。通过将单价阳离子浓度从0.154 M降至0.034 M以及向反应混合物中添加蛋白质，可以改变这种敏感性。在劳氏肉瘤病毒和狒狒内源性C型病毒中检测到逆转录酶，但在梅森- Pfizer猴D型病毒中未检测到，这表明它们有相似的要求。
