在两种功能不相关的毒素中发现的一种共同抗原决定簇。
A common antigenic determinant found in two functionally unrelated toxins.
作者信息
Gariepy J, O'Hanley P, Waldman S A, Murad F, Schoolnik G K
出版信息
J Exp Med. 1984 Oct 1;160(4):1253-8. doi: 10.1084/jem.160.4.1253.
Abstract
The heat-stable enterotoxin ST Ib produced by enterotoxigenic E. coli strains shares a sequence homology with the sea snail neurotoxin, conotoxin GI. Rabbit antisera were raised against synthetic analogs of these toxins and to a six-residue peptide representing the region common to both toxins. Results from enzyme-linked immunosorbent assays indicate that the homologous region of both toxins represents part of their antigenic site. The lack of cross-reactivity exhibited by the six-residue common domain with serum directed against either toxin suggests that this region probably retains a similar conformation in the intact toxins but not in the isolated fragment.
摘要
产肠毒素大肠杆菌菌株产生的热稳定肠毒素ST Ib与海螺神经毒素芋螺毒素GI具有序列同源性。用这些毒素的合成类似物以及代表两种毒素共同区域的六肽制备了兔抗血清。酶联免疫吸附测定结果表明，两种毒素的同源区域代表了它们抗原位点的一部分。六个残基的共同结构域与针对任何一种毒素的血清均无交叉反应，这表明该区域在完整毒素中可能保留相似的构象，但在分离片段中则不然。
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