Itabashi M, Aoyama M, Kono M, Tanaka N
Gan No Rinsho. 1984 Aug;30(10):1293-8.
Administration of hematoporphyrin derivative (HPD) with the association of laser beam irradiation has recently been employed for the diagnosis and treatment of cancer. This methodology is based on the tumor affinity of HPD and the phenomenon of fluorescence generation under the specific wave laser beam. Furthermore, laser irradiation induces a photochemical reaction of HPD, which may kill the HPD uptake cancer cells. Utilizing the electron spin resonance (ESR) that produces HPD radicals, we measured the level of radicals in the lymphocytes separated from the peripheral blood of patients with cancer of various types and sites and that from normal controls, as well as in the culture cancer cells (K-562). The level of radicals in the lymphocytes from the cancer-bearing patients and culture cancer cells was found to be significantly elevated as compared with that in the lymphocytes of normal controls and patients with noncancerous conditions such as SLE, hepatic disease, etc. This procedure appears to be very useful for the diagnosis of cancer, and this is the first report in the world.
血卟啉衍生物（HPD）与激光束照射联合应用，近来已用于癌症的诊断和治疗。该方法基于HPD对肿瘤的亲和力以及在特定波长激光束照射下产生荧光的现象。此外，激光照射可引发HPD的光化学反应，这可能会杀死摄取了HPD的癌细胞。利用产生HPD自由基的电子自旋共振（ESR），我们测量了从各类不同类型和部位癌症患者外周血以及正常对照者分离出的淋巴细胞中的自由基水平，以及培养的癌细胞（K - 562）中的自由基水平。结果发现，与正常对照者以及患有如系统性红斑狼疮、肝病等非癌性疾病患者的淋巴细胞相比，癌症患者淋巴细胞和培养癌细胞中的自由基水平显著升高。这一检测程序似乎对癌症诊断非常有用，这是世界上的首次报道。
