Tanaka M, Sato S, Miyatake T
Jpn J Exp Med. 1984 Feb;54(1):9-15.
Although cross-reactivity between T cells and nervous tissue elements has been reported previously, the antigenic determinants of the cross-reactivity are still unknown. In this study, using immunofluorescence, we demonstrate that the same antigenic determinant as myelin-associated glycoprotein (MAG) exists on the cell surface of mononuclear cells in human peripheral blood. MAG positive cells of thirteen healthy individuals were from 6.1 percent to 14.0 percent (mean +/- standard deviation: 9.1 +/- 2.0% of total mononuclear cells in human peripheral blood). The percentage of MAG positive cells was diminished from 9.3% to 1.3% by preincubation with Leu-7, a monoclonal antibody against human natural killer cells. The percentage of Leu-7 positive cells was not changed by preincubation with anti-MAG antiserum. MAG may be one of the specific antigens which exist in both human peripheral blood mononuclear cells and nervous tissues, and may also be a marker of human natural killer cells.
尽管此前已有关于T细胞与神经组织成分之间交叉反应性的报道，但这种交叉反应性的抗原决定簇仍不清楚。在本研究中，我们使用免疫荧光法证明，人类外周血单个核细胞的细胞表面存在与髓鞘相关糖蛋白（MAG）相同的抗原决定簇。13名健康个体的MAG阳性细胞占人类外周血单个核细胞总数的6.1%至14.0%（平均值±标准差：9.1±2.0%）。用抗人类自然杀伤细胞的单克隆抗体Leu-7预孵育后，MAG阳性细胞的百分比从9.3%降至1.3%。用抗MAG抗血清预孵育后，Leu-7阳性细胞的百分比没有变化。MAG可能是人类外周血单个核细胞和神经组织中都存在的特异性抗原之一，也可能是人类自然杀伤细胞的标志物。
