Moore G R, Traugott U, Farooq M, Norton W T, Raine C S
Lab Invest. 1984 Oct;51(4):416-24.
Alterations in the effect of a known encephalitogenic dose of myelin basic protein (MBP) when inoculated in combination with various myelin lipids have been examined in guinea pigs. A previous study demonstrated that, when MBP was given with galactocerebroside, it produced an acute autoimmune encephalomyelitis similar to that induced by whole white matter in which both inflammation and demyelination were features of the central nervous system lesions. MBP alone, on the other hand, resulted in inflammation only, without demyelination. The present study examined combinations of MBP with the myelin lipids galactocerebroside, sulfatide, ethanolamine phosphoglycerides, and serine phosphoglycerides. The lipids were given with or without MBP, in the same ratio as in intact central nervous system myelin, and were emulsified with complete Freund's adjuvant. An additional group received galactocerebroside and bovine serum albumin in complete Freund's adjuvant. These groups were compared with animals receiving either bovine white matter or MBP in complete Freund's adjuvant. Clinical autoimmune encephalomyelitis was observed in animals receiving bovine white matter, MBP, and all lipid-MBP emulsions; the bovine white matter, galactocerebroside/MBP, sulfatide/MBP, and ethanolamine phosphoglycerides/MBP groups demonstrated central nervous system lesions with a similar picture consisting of inflammation with demyelination, whereas inflammation without demyelination was seen in the MBP and serine phosphoglycerides/MBP groups. Thus, the addition of myelin lipids to MBP leads to the augmentation of demyelination in autoimmune encephalomyelitis lesions in the guinea pig. This might suggest that the immune response against MBP is enhanced by other myelin components. The relevance of these findings to human demyelinating disorders is discussed.
在豚鼠中，研究了已知致脑炎性剂量的髓鞘碱性蛋白（MBP）与各种髓磷脂脂质联合接种时其效果的改变。先前的一项研究表明，当MBP与半乳糖脑苷脂一起给予时，会引发一种急性自身免疫性脑脊髓炎，类似于全白质诱导的疾病，其中炎症和脱髓鞘都是中枢神经系统病变的特征。另一方面，单独的MBP仅导致炎症，而无脱髓鞘。本研究检测了MBP与髓磷脂脂质半乳糖脑苷脂、硫脂、乙醇胺磷酸甘油酯和丝氨酸磷酸甘油酯的组合。脂质与MBP一起或不与MBP一起给予，比例与完整中枢神经系统髓磷脂中的相同，并用完全弗氏佐剂乳化。另一组在完全弗氏佐剂中接受半乳糖脑苷脂和牛血清白蛋白。将这些组与在完全弗氏佐剂中接受牛白质或MBP的动物进行比较。在接受牛白质、MBP和所有脂质-MBP乳剂的动物中观察到临床自身免疫性脑脊髓炎；牛白质、半乳糖脑苷脂/MBP、硫脂/MBP和乙醇胺磷酸甘油酯/MBP组表现出中枢神经系统病变，其特征相似，包括伴有脱髓鞘的炎症，而在MBP和丝氨酸磷酸甘油酯/MBP组中则可见无脱髓鞘的炎症。因此，向MBP中添加髓磷脂脂质会导致豚鼠自身免疫性脑脊髓炎病变中脱髓鞘的增加。这可能表明针对MBP的免疫反应会被其他髓磷脂成分增强。讨论了这些发现与人类脱髓鞘疾病的相关性。
