Kapadia S E, de Lanerolle N C
Neuroscience. 1984 Aug;12(4):1229-42. doi: 10.1016/0306-4522(84)90017-4.
The localization of substance P-like immunoreactivity in the interpeduncular nucleus using the peroxidase-antiperoxidase technique, revealed that the median region of the interpeduncular nucleus was one area rich in substance P-like immunoreactive processes. The ultrastructural characteristics of these substance P-like immunoreactive processes and their organization within the middle zone of the median region of the interpeduncular nucleus was studied. Substance P-like immunoreactivity was found in the perikaryon of small neurons, and in proximal and small dendrites. The substance P-like immunoreactive cell bodies and proximal dendrites receive a variety of unlabeled synaptic terminals. The immunoreactive small dendrites usually formed the central component of a "rosette"-like formation with unlabeled terminals. A few immunoreactive small unmyelinated axons and boutons were also present in the neuropil. The substance P-like immunoreactive boutons contained mainly small round vesicles with some large dense-core vesicles. These substance P-like immunoreactive boutons were presynaptic to unlabeled dendritic profiles, and frequently to substance P-like immunoreactive dendritic profiles. They were also seen in apposition to unlabeled boutons. Substance P-like immunoreactive boutons were not found to synapse with the crest-like dendritic processes in this part of the interpeduncular nucleus. It is suggested on the basis of morphological features, that some of the unlabeled terminals synapsing on substance P-like immunoreactive dendrities, may be cholinergic in nature.
运用过氧化物酶-抗过氧化物酶技术对脚间核内P物质样免疫反应性进行定位研究，结果显示脚间核的中间区域是富含P物质样免疫反应性神经突起的一个区域。对这些P物质样免疫反应性神经突起在脚间核中间区域中区的超微结构特征及其组织情况进行了研究。在小神经元的胞体、近端和小的树突中发现了P物质样免疫反应性。P物质样免疫反应性细胞体和近端树突接受多种无标记的突触终末。免疫反应性小树突通常与无标记终末一起形成“玫瑰花结”样结构的中心部分。在神经毡中也存在一些免疫反应性小的无髓轴突和终扣。P物质样免疫反应性终扣主要含有小圆形囊泡以及一些大的致密核心囊泡。这些P物质样免疫反应性终扣位于无标记树突轮廓的突触前，并且经常位于P物质样免疫反应性树突轮廓的突触前。它们也见于与无标记终扣并列的位置。在脚间核的这一部分未发现P物质样免疫反应性终扣与嵴状树突突起形成突触。基于形态学特征推测，一些与P物质样免疫反应性树突形成突触的无标记终末可能是胆碱能性质的。
