3,3',5,5'-四甲基联苯胺/过氧化氢染色对于通过凝胶电泳分离的血红素蛋白不具有特异性。

3,3',5,5'-tetramethylbenzidine/H2O2 staining is not specific for heme proteins separated by gel electrophoresis.

作者信息

Miller D J, Nicholas D J

出版信息

Anal Biochem. 1984 Aug 1;140(2):577-80. doi: 10.1016/0003-2697(84)90209-4.

PMID:6207749

Abstract

Staining of sodium dodecyl sulfate or lithium dodecyl sulfate gels with 3,3',5,5'-tetramethylbenzidine (TMBZ)/H2O2 after electrophoresis has frequently been used as a specific method of detecting heme proteins. That TMBZ is an electron donor for O2 reduction by the nonheme-soluble cytochrome oxidase/nitrite reductase from Nitrosomonas europaea is now shown; this protein is detected by the TMBZ/H2O2 method. A method for the determination of TMBZ oxidase activity is given; hence, the detection of artifactual staining due to proteins of this type is possible.

摘要

电泳后用3,3',5,5'-四甲基联苯胺（TMBZ）/H₂O₂对十二烷基硫酸钠或十二烷基硫酸锂凝胶进行染色，常被用作检测血红素蛋白的一种特定方法。现已表明，TMBZ是欧洲亚硝化单胞菌非血红素可溶性细胞色素氧化酶/亚硝酸盐还原酶将O₂还原的电子供体；这种蛋白质可通过TMBZ/H₂O₂方法检测到。给出了一种测定TMBZ氧化酶活性的方法；因此，可以检测到这类蛋白质引起的人为染色。