通过免疫细胞化学法检测U1和U2小核核糖核蛋白的共定位。
Colocalization of U1 and U2 small nuclear RNPs by immunocytochemistry.
作者信息
Spector D L
出版信息
Biol Cell. 1984;51(1):109-12. doi: 10.1111/j.1768-322x.1984.tb00289.x.
Abstract
The in situ localization of U1 and U2 snRNPs was examined, using autoantibodies directed against each of these snRNPs, by immunofluorescence and immunoelectron microscopy. This study has shown U1 and U2 snRNPs to colocalize in the nuclei of PtK2 cells. Thirty to fifty immunostained clusters were observed per interphase nucleus. This study suggests these nuclear protein clusters to be the sites of RNA processing.
摘要
利用针对这些小核核糖核蛋白（snRNP）的自身抗体，通过免疫荧光和免疫电子显微镜检查了U1和U2 snRNP的原位定位。这项研究表明，U1和U2 snRNP在PtK2细胞核中共定位。每个间期核中观察到30至50个免疫染色簇。这项研究表明，这些核蛋白簇是RNA加工的场所。
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