Takano Y, Loewy A D
Brain Res. 1984 Oct 8;311(1):144-7. doi: 10.1016/0006-8993(84)91407-0.
[3H]substance P binding was studied in the intermediolateral cell column and striatum in the rat using slide-mounted sections. The intermediolateral cell column had a single high affinity binding component with a dissociation constant, Kd = 1.45 nM and the number of sites, Bmax = 18.1 fmol per mg protein. The striatum had a Kd = 0.77 nM and a Bmax = 23.5 fmol per mg protein. The relative potency of various substance P-like tachykinins in displacing [3H]substance P suggested that both these areas may contain a substance P-P (for physalaemin) receptor subtype. (D-Pro4, D-Trp7,9)substance P(4-11), a substance P antagonist, has a relatively low affinity (micromolar range) in both these areas.
利用载玻片固定的切片，对大鼠中间外侧细胞柱和纹状体中的[3H]P物质结合进行了研究。中间外侧细胞柱有一个单一的高亲和力结合成分，解离常数Kd = 1.45 nM，位点数量Bmax = 每毫克蛋白质18.1 fmol。纹状体的Kd = 0.77 nM，Bmax = 每毫克蛋白质23.5 fmol。各种P物质样速激肽在置换[3H]P物质方面的相对效力表明，这两个区域可能都含有P物质-P（针对雨蛙肽）受体亚型。P物质拮抗剂（D-脯氨酸4，D-色氨酸7,9）P物质（4-11）在这两个区域的亲和力相对较低（微摩尔范围）。
