Tomizawa J
Cell. 1984 Oct;38(3):861-70. doi: 10.1016/0092-8674(84)90281-2.
A transcript (RNA II) from the ColE1 primer promoter forms a persistent hybrid with the template DNA near the replication origin. The hybridized RNA II is cleaved by RNAase H to form the primer. Binding of RNA I to RNA II inhibits the hybridization and thus prevents primer formation. The binding is demonstrated by identification of the product. Analyses of the product with RNAase T1 revealed that pairing initiates at or near the 5' end of RNA I and propagates toward its 3' end. Removal of five nucleotides from the 5' end of RNA I greatly reduces its binding activity. Prior to initiation of pairing, RNA I interacts with RNA II at their single-stranded loops. This interaction is transient but facilitates complete pairing along the entire length of RNA I that initiates from the 5'-end region.
来自ColE1引物启动子的转录本（RNA II）与复制起点附近的模板DNA形成持久的杂交体。杂交的RNA II被RNA酶H切割形成引物。RNA I与RNA II的结合抑制杂交，从而阻止引物形成。通过鉴定产物来证明这种结合。用RNA酶T1对产物进行分析表明，配对从RNA I的5'端或其附近开始，并向其3'端延伸。从RNA I的5'端去除五个核苷酸会大大降低其结合活性。在配对开始之前，RNA I与RNA II在它们的单链环处相互作用。这种相互作用是短暂的，但有助于从5'端区域开始沿着RNA I的整个长度进行完全配对。
