Tomizawa J, Som T
Cell. 1984 Oct;38(3):871-8. doi: 10.1016/0092-8674(84)90282-4.
RNA I prevents a transcript (RNA II) from the ColE1 primer promoter to form a hybrid with the template DNA and thereby inhibits formation of primer for DNA replication. Binding of RNA I to RNA II is responsible for the inhibition. The Rom protein, a plasmid-specified 63 amino acid protein, enhances the inhibitory effect of RNA I on primer formation by enhancing the binding of RNA I to RNA II. In vivo, RNA I controls plasmid copy number and incompatibility and inhibits expression of a galK gene fused to the primer promoter. The rom gene modulates these activities of RNA I. These functions of the rom gene can be explained by alteration of the binding of RNA I to RNA II by the Rom protein.
RNA I可阻止来自ColE1引物启动子的转录本（RNA II）与模板DNA形成杂交体，从而抑制DNA复制引物的形成。RNA I与RNA II的结合导致了这种抑制作用。Rom蛋白是一种由质粒编码的含63个氨基酸的蛋白质，它通过增强RNA I与RNA II的结合来增强RNA I对引物形成的抑制作用。在体内，RNA I控制质粒拷贝数和不相容性，并抑制与引物启动子融合的galK基因的表达。rom基因调节RNA I的这些活性。rom基因的这些功能可以通过Rom蛋白改变RNA I与RNA II的结合来解释。
