针对人肺癌细胞系SK-LC-3的小鼠单克隆IgM抗体，对H(O)血型抗原有特异性。

Mouse monoclonal IgM antibody against human lung cancer line SK-LC-3 with specificity for H(O) blood group antigen.

作者信息

Anger B R, Lloyd K O, Oettgen H F, Old L J

出版信息

Hybridoma. 1982;1(2):139-47. doi: 10.1089/hyb.1.1982.1.139.

DOI:10.1089/hyb.1.1982.1.139

PMID:6208122

Abstract

Spleen cells from mice immunized with the human lung cancer line SK-LC-3 were fused with mouse NS-1 myeloma cells. One of the hybrid clones produced a monoclonal IgM antibody (designated F-3), detected with antimouse Ig-MHA and hemagglutination assays. This antibody was completely absorbed by O red cells and completely inhibited by low concentrations of H(O) glycoproteins and hog mucin (A + H). Bombay (Oh) red cells completely failed to absorb F-3 activity even after treatment with neuraminidase. A1, A2, A1B, A2B, and B red cells and A- and B-glycoproteins were less effective in absorbing or inhibiting F-3 activity. Other glycoproteins (including those having Lea or blood group precursor structures) showed little or no inhibitory activity. Serum from nude mice carrying F-3 hybridoma agglutinated O and A2 red cells at a titer of 1:40,000 and 1:640, respectively. A1, A1B, A2B, and B red cells were agglutinated with titers of 1:80 or less. Monoclonal antibody F-3 is, therefore, highly specific for H(O) blood group determinants.

摘要

用人类肺癌细胞系SK-LC-3免疫的小鼠脾细胞与小鼠NS-1骨髓瘤细胞融合。其中一个杂交克隆产生了一种单克隆IgM抗体（命名为F-3），通过抗小鼠Ig-MHA和血凝试验检测到。该抗体完全被O型红细胞吸收，并被低浓度的H(O)糖蛋白和猪粘蛋白（A + H）完全抑制。孟买（Oh）型红细胞即使在用神经氨酸酶处理后也完全不能吸收F-3活性。A1、A2、A1B、A2B和B型红细胞以及A-和B-糖蛋白在吸收或抑制F-3活性方面效果较差。其他糖蛋白（包括具有Lea或血型前体结构的糖蛋白）显示出很少或没有抑制活性。携带F-3杂交瘤的裸鼠血清分别以1:40,000和1:640的效价凝集O型和A2型红细胞。A1、A1B、A2B和B型红细胞的凝集效价为1:80或更低。因此，单克隆抗体F-3对H(O)血型决定簇具有高度特异性。