Kimmel K A, Carey T E, Judd W J, McClatchey K D
J Natl Cancer Inst. 1986 Jan;76(1):9-19.
The IgM monoclonal antibody G10 was raised against the human squamous cell carcinoma (SCC) cell line UM-SCC-1. In initial screening against cultured cells, G10 bound to 2 SCC lines (UM-SCC-1 and UM-SCC-13) and 1 pancreatic carcinoma line (UM-PAd-1) but not to cultured fibroblasts (WI-38), ovarian carcinoma cells (SK-OV-3), or malignant melanoma cells (SK-MEL-28 and MeWo). In subsequent tests against cultured cell lines, G10 gave positive reactions with 30 of 33 SCC lines but only 4 of 29 non-SCC lines. The non-SCC lines that bound G10 were UM-PAd-1, 2 transitional cell carcinoma lines (T24 and RT4), and 1 melanoma line (SK-MEL-22). When tested against cultures derived from normal skin or mucosa, G10 was reactive with the epitheloid squamous cells but not with the fibroblasts in each culture. The antigen defined by antibody G10 was stable to fixation with Formalin, and its distribution in tissue sections was examined with the use of immunoperoxidase assays. All SCC biopsy specimens examined in this way were reactive with antibody G10. In similar tests against sections of fixed normal tissues, G10 stained the superficial squamous cells of the epidermis and the basal and suprabasal layers of mucosal squamous epithelial cells from the esophagus. All layers of the laryngeal epithelium were positive. Endothelial cells and certain glandular cells were also positive for G10 binding. G10 agglutinated human red blood cells of all blood groups except those from individuals of the Bombay group (Oh) who lack the H blood group determinant. Against defined oligosaccharides, G10 bound strongly only to the monofucosyl H type 2 structure and was slightly cross-reactive with the synthetic difucosyl H type 2 or Y structure. These results are consistent with previous reports of blood group antigen tissue distribution and indicate that the H type 2 determinant is expressed by all or nearly all mucosal squamous cancers. Less frequent expression by cells of other tumor types may correlate with tissue-specific activation of the H gene-specified fucosyltransferase.
IgM单克隆抗体G10是针对人鳞状细胞癌（SCC）细胞系UM-SCC-1产生的。在对培养细胞的初步筛选中，G10与2种SCC细胞系（UM-SCC-1和UM-SCC-13）和1种胰腺癌细胞系（UM-PAd-1）结合，但不与培养的成纤维细胞（WI-38）、卵巢癌细胞（SK-OV-3）或恶性黑色素瘤细胞（SK-MEL-28和MeWo）结合。在随后对培养细胞系的测试中，G10与33种SCC细胞系中的30种呈阳性反应，但与29种非SCC细胞系中只有4种呈阳性反应。与G10结合的非SCC细胞系是UM-PAd-1、2种移行细胞癌细胞系（T24和RT4）和1种黑色素瘤细胞系（SK-MEL-22）。当对来自正常皮肤或黏膜的培养物进行测试时，G10与各培养物中的上皮样鳞状细胞反应，但不与成纤维细胞反应。抗体G10所定义的抗原对福尔马林固定稳定，并使用免疫过氧化物酶测定法检查其在组织切片中的分布。以这种方式检查的所有SCC活检标本均与抗体G10反应。在对固定正常组织切片的类似测试中，G10对表皮的表层鳞状细胞以及食管黏膜鳞状上皮细胞的基底层和基底上层进行染色。喉上皮的所有层均呈阳性。内皮细胞和某些腺细胞对G10结合也呈阳性。G10凝集除孟买血型（Oh）个体（缺乏H血型决定簇）以外的所有血型的人红细胞。针对特定的寡糖，G10仅与单岩藻糖基化H2型结构强烈结合，并与合成的双岩藻糖基化H2型或Y结构有轻微交叉反应。这些结果与先前关于血型抗原组织分布的报道一致，并表明H2型决定簇在所有或几乎所有黏膜鳞状癌中均有表达。其他肿瘤类型细胞中表达频率较低可能与H基因指定的岩藻糖基转移酶的组织特异性激活有关。
