Roth J, Lucocq J M, Charest P M
J Histochem Cytochem. 1984 Nov;32(11):1167-76. doi: 10.1177/32.11.6208237.
The development of a cytochemical affinity technique for the demonstration of sialic acid residues by light and electron microscopy is reported. The lectin from the slug Limax flavus, with its narrow specificity for N-acetyl- and N-glycolylneuraminic acid, was applied to tissue sections. Subsequently fetuin-gold complexes were used to visualize the tissue-bound lectin. Different cytochemical controls, including sugar inhibition tests, neuraminidase digestion, the use of fetuin-gold complexes alone, or acid hydrolysis of sections, proved the specificity of the technique. Postembedding staining was performed on frozen, paraffin, or semithin resin sections for light microscopy and on thin sections from low temperature Lowicryl K4M-embedded material for electron microscopy. The distribution of sialic acid residues in rat pancreas, liver, and colonic mucosa was investigated.
本文报道了一种用于通过光学显微镜和电子显微镜显示唾液酸残基的细胞化学亲和技术的开发。来自黄蛞蝓的凝集素，对N-乙酰神经氨酸和N-糖基神经氨酸具有狭窄的特异性，应用于组织切片。随后使用胎球蛋白-金复合物来可视化与组织结合的凝集素。不同的细胞化学对照，包括糖抑制试验、神经氨酸酶消化、单独使用胎球蛋白-金复合物或切片的酸水解，证明了该技术的特异性。对冷冻、石蜡或半薄树脂切片进行包埋后染色用于光学显微镜检查，对低温Lowicryl K4M包埋材料的超薄切片进行电子显微镜检查。研究了大鼠胰腺、肝脏和结肠黏膜中唾液酸残基的分布。
