Obinata A, Takata K, Kawada M, Hirano H, Endo H
J Invest Dermatol. 1984 Nov;83(5):363-9. doi: 10.1111/1523-1747.ep12264632.
Our previous study showed that hydrocortisone (10(-8) M) induced epidermal alpha-type keratinization in 13-day-old chick embryonic tarsometatarsal skin cultured in a chemically defined medium. In this study, we show that typical glucocorticoid receptor is present in epidermal cells of chick embryonic cultured skin and that alpha-type keratinization of epidermal cells is completely prevented by the coexistence of a 100-fold excess concentration of progesterone with glucocorticoid. The physiologic importance of the receptor is suggested by some correlation between the ability of progesterone to block both the differentiation-induction and the binding of active glucocorticoid to the receptor.
我们之前的研究表明，在化学限定培养基中培养的13日龄鸡胚跗跖皮肤中，氢化可的松（10^(-8) M）可诱导表皮α型角质化。在本研究中，我们发现鸡胚培养皮肤的表皮细胞中存在典型的糖皮质激素受体，并且当孕酮浓度比糖皮质激素高100倍时，可完全阻止表皮细胞的α型角质化。孕酮能够同时阻断分化诱导以及活性糖皮质激素与受体的结合，这两者之间的某种关联提示了该受体的生理重要性。
