Valladares L E, Ronco A M
J Steroid Biochem. 1984 Oct;21(4):443-6. doi: 10.1016/0022-4731(84)90309-1.
Luteotrophic hormone acts on testicular interstitial cells, promoting the activation of several cellular events that culminate in steroids synthesis. Since the interstitial tissue include several cell types, purified Leydig cells were used in this work. Isolated interstitial cells from immature rats were purified through a 0-40% metrizamide gradient. Either LH, HCG or Bt2-cAMP significantly stimulated the incorporation of [3H]uridine into RNA, when compared to control. The effect of HCG on RNA synthesis was developed within 30 min after the addition of the hormone and was dose-dependent. The maximum effect was attained with 10 mIU/ml of HCG. These results indicate that HCG/LH or Bt2-cAMP but not FSH, promote an acute stimulation of RNA synthesis by Leydig cells from immature rats.
黄体生成素作用于睾丸间质细胞，促进一系列细胞活动的激活，最终导致类固醇的合成。由于间质组织包含多种细胞类型，因此本研究使用了纯化的睾丸间质细胞。通过0-40%的甲泛影酰胺梯度对未成熟大鼠分离出的间质细胞进行纯化。与对照组相比，促黄体生成素（LH）、人绒毛膜促性腺激素（HCG）或双丁酰环磷腺苷（Bt2-cAMP）均能显著刺激[3H]尿苷掺入RNA。HCG对RNA合成的作用在添加激素后30分钟内显现，且呈剂量依赖性。10 mIU/ml的HCG可达到最大效应。这些结果表明，HCG/LH或Bt2-cAMP而非促卵泡生成素（FSH），可促进未成熟大鼠睾丸间质细胞对RNA合成的急性刺激。
