McLoughlin J L, Cantrill R C
Neurosci Lett. 1984 Aug 24;49(1-2):175-80. doi: 10.1016/0304-3940(84)90156-3.
Total particulate material prepared by homogenization in water and centrifugation at 100,000 g for 60 min from the brains of normal and vitamin B12-deficient fruit bats was fractionated on linear sucrose gradients (0.1 M-1.4 M sucrose). Animals were made vitamin B12-deficient by dietary deprivation or as a result of exposure to nitrous oxide. Based on absorbance at 280 nm three peaks were seen in material derived from the B12-deficient fruit bat brain and only two peaks in the normal animal. Myelin proteins were observed over a larger range of molarities of sucrose in the deficient brain than in the control tissue. Animals rendered vitamin B12-deficient by nitrous oxide treatment showed membrane protein patterns similar to those observed in the control animal.
通过在水中匀浆并在100,000 g下离心60分钟，从正常和维生素B12缺乏的果蝠大脑中制备的总颗粒物质，在线性蔗糖梯度（0.1 M - 1.4 M蔗糖）上进行分级分离。通过饮食剥夺或暴露于一氧化二氮使动物缺乏维生素B12。基于280 nm处的吸光度，在维生素B12缺乏的果蝠大脑来源的物质中观察到三个峰，而在正常动物中仅观察到两个峰。与对照组织相比，在缺乏维生素B12的大脑中，髓磷脂蛋白在更大范围的蔗糖摩尔浓度中被观察到。通过一氧化二氮处理使维生素B12缺乏的动物显示出与对照动物中观察到的膜蛋白模式相似。
