Kronquist K E, Crandall B F, Cosico L G
Tumour Biol. 1984;5(1):15-31.
We compared the two-dimensional polypeptide pattern of donor-matched samples of mid-gestational amniotic fluid and maternal plasma to identify fetally-derived polypeptides in the amniotic fluid. Ten major polypeptide groups, ranging in molecular mass from 14.6 to 225 Kilodaltons (K), were consistently present in amniotic fluid that were not detected in maternal plasma. Three of these polypeptide complexes have been identified: one is alphafetoprotein, the second is a cellular form of fibronectin, and the third is beta 2-microglobulin. We searched for the remaining seven polypeptides in fetal serum and urine to confirm their fetal origin. Urine contained two polypeptides at 30.5K and 25.5K with mobilities identical to species found in amniotic fluid; the 25.5K polypeptide was also present in cord serum. Possible sources for the remaining 5 components may be the fetal respiratory and gastrointestinal tracts, integument, membranes (amnion and chorion) or the uterus. In addition, developmental changes in amniotic fluid polypeptides were studied by comparing samples obtained at mid-gestation and at term. Three proteins characteristic of mid-gestational amniotic fluid with masses of 185.8K, 38.2K and 25.4K were not detected or were present only at trace levels at term; and new polypeptide groups at approximately 51K, and 18K were observed in late gestation.
我们比较了孕中期羊水与母体血浆供体匹配样本的二维多肽图谱，以鉴定羊水中胎儿来源的多肽。在羊水中始终存在10个主要多肽组，其分子量范围为14.6至225千道尔顿（K），而在母体血浆中未检测到。已鉴定出其中三种多肽复合物：一种是甲胎蛋白，第二种是细胞形式的纤连蛋白，第三种是β2-微球蛋白。我们在胎儿血清和尿液中寻找其余七种多肽，以确认它们的胎儿来源。尿液中含有两种分子量分别为30.5K和25.5K的多肽，其迁移率与羊水中发现的种类相同；25.5K的多肽也存在于脐血血清中。其余5种成分的可能来源可能是胎儿的呼吸道和胃肠道、体被、胎膜（羊膜和绒毛膜）或子宫。此外，通过比较孕中期和足月时获得的样本，研究了羊水多肽的发育变化。孕中期羊水中三种特征性蛋白质，分子量分别为185.8K、38.2K和25.4K，在足月时未检测到或仅以痕量水平存在；在妊娠后期观察到了约51K和18K的新多肽组。
