环氧树脂切片的氧化对苯二胺染色

Oxidized p-phenylenediamine staining of epoxy resin sections.

作者信息

Stockert J C, Armas-Portela R, Colman O D, Ferrer J M, Tato A

出版信息

Z Naturforsch C Biosci. 1984 Jul-Aug;39(7-8):835-6. doi: 10.1515/znc-1984-7-824.

DOI:10.1515/znc-1984-7-824

PMID:6208701

Abstract

Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable electron opacity in PPD positive structures.

摘要

将戊二醛固定、环氧树脂包埋的动物组织的半薄切片和薄切片用氧化对苯二胺（PPD）溶液处理。该方法适用于在光学显微镜下显示组织的一般形态，在一些含聚阴离子的成分中显示出高染色程度。用氯化金溶液对薄切片进行后处理，可使PPD阳性结构产生相当大的电子不透明度。