Müller W A
Angew Parasitol. 1984 Aug;25(3):120-6.
Detection of antigenspecific suppressor cells in Toxoplasma infection of man. Investigations adapted to demonstrate the action of antigen-specific suppressor cells were carried out in subjects with acute toxoplasmosis (n = 5) and latent infections (n = 16) and compared to non-infected controls (n = 11). The investigations were carried out in vitro in the form of autologous one-way lymphocyte mixed culture. Suppressor cells were activated by toxoplasma lysate antigen (325 micrograms/ml) and, for comparison, by concanavalin A (10 micrograms/ml). Responder cells were stimulated by toxoplasma lysate antigen (200 micrograms/ml). In the acute cases, antigen-specific suppressor cells were identified which caused the lymphocyte transformation to be suppressed (tritium thymidine incorporation) to the same extent as did the mitogen-activated cells. The subjects with latent infections revealed a statistically significant difference (p less than 0.01) between the means of the percentage suppression of suppressor cells activated by toxoplasma lysate antigen or concanavalin A, suggesting a diminishing suppressive action of antigen-specific cells at this stage of infection. In addition to the effect on phagosome formation in macrophages, the activation of suppressor cells too should be considered as a factor of virulence for Toxoplasma gondii.
人体弓形虫感染中抗原特异性抑制细胞的检测。在急性弓形虫病患者（n = 5）和潜伏感染患者（n = 16）中开展了旨在证明抗原特异性抑制细胞作用的研究，并与未感染的对照组（n = 11）进行比较。研究以自体单向淋巴细胞混合培养的形式在体外进行。抑制细胞用弓形虫裂解物抗原（325微克/毫升）激活，作为对照，也用刀豆蛋白A（10微克/毫升）激活。应答细胞用弓形虫裂解物抗原（200微克/毫升）刺激。在急性病例中，鉴定出抗原特异性抑制细胞，其导致淋巴细胞转化（氚胸腺嘧啶掺入）受到抑制，抑制程度与丝裂原激活的细胞相同。潜伏感染患者中，用弓形虫裂解物抗原或刀豆蛋白A激活的抑制细胞的抑制百分比均值之间存在统计学显著差异（p < 0.01），表明在感染的这个阶段抗原特异性细胞的抑制作用在减弱。除了对巨噬细胞吞噬体形成的影响外，抑制细胞的激活也应被视为刚地弓形虫毒力的一个因素。
