用类风湿性关节炎患者的循环免疫复合物对豚鼠进行免疫接种。
Immunisation of guinea-pigs with circulating immune complexes from patients with rheumatoid arthritis.
作者信息
Hack C E, Lim H G, Aalberse R C
出版信息
Ann Rheum Dis. 1984 Oct;43(5):749-54. doi: 10.1136/ard.43.5.749.
Sixteen guinea-pigs were immunised with immune complexes isolated from serum of nine patients with rheumatoid arthritis. The resulting antisera were analysed by radioimmunoassays. All guinea-pig sera were extensively absorbed with normal human serum. After this absorption eight guinea-pig sera contained antibodies specific for immune complexes isolated from the sera of three patients. One of these antisera reacted not only with immune complexes (and serum) from the corresponding patient but also with immune complexes (and sera) from other patients with rheumatoid arthritis. The antigen(s) to which the guinea-pig antibodies were directed sedimented as IgM, and they bound to IgG Sepharose. Therefore the guinea-pig sera were absorbed with IgM-rheumatoid factors isolated from the serum of the corresponding patient. After this absorption, the guinea-pig sera had lost their reactivity with immune complexes. We conclude that these antisera did not detect an exogenous antigen in immune complexes from patients with rheumatoid arthritis. The positive reactions found were due to antibodies specific for (idiotypic?) antigenic determinants on IgM-rheumatoid factors.
用从9例类风湿性关节炎患者血清中分离出的免疫复合物对16只豚鼠进行免疫。通过放射免疫测定法分析所得抗血清。所有豚鼠血清都用正常人血清进行了广泛吸收。吸收后，8只豚鼠血清含有针对从3例患者血清中分离出的免疫复合物的特异性抗体。其中一种抗血清不仅与相应患者的免疫复合物（和血清）发生反应，还与其他类风湿性关节炎患者的免疫复合物（和血清）发生反应。豚鼠抗体所针对的抗原以IgM形式沉淀，并且它们与IgG琼脂糖结合。因此，用从相应患者血清中分离出的IgM类风湿因子对豚鼠血清进行吸收。吸收后，豚鼠血清失去了与免疫复合物的反应性。我们得出结论，这些抗血清未检测到类风湿性关节炎患者免疫复合物中的外源性抗原。所发现的阳性反应是由于对IgM类风湿因子上（独特型？）抗原决定簇具有特异性的抗体所致。
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